sam68 wt lentivirus expression vector Search Results


pcdna3 ha sam68 wt vector  (Addgene inc)
93
Addgene inc pcdna3 ha sam68 wt vector
a) Schematic illustration of the construct used for expressing RhoBAST-tagged G FP mRNA. b) Confocal images of HEK293T cells expressing GFP-RhoBAST 16 or GFP (control) mRNA incubated with SpyRho (100 nM) for 1 h before imaging. c) Normalized fluorescence profile along the dashed line in panel b (lower le). d) Schematic illustration of the constructs used for expressing CGG-containing FMR1-GFP mRNAs fused to 16 repeats of RhoBAST and <t>HaloTag7-Sam68.</t> e) Confocal images of live Cos7 cells expressing CGG99-FMR1-GFP-RhoBAST 16, CGG99-FMR1-GFP or GFP (control) mRNA and HaloTag7-Sam68 fusion protein (plasmid ratio 10:1) incubated with SpyRho (100 nM) and MaP700-Halo (200 nM, SiR channel) for 1 h before imaging. f) Normalized fluorescence profiles along the dashed lines shown in e). The fluorescence in the TMR channel was normalized to the highest value detected for CGG99-FMR1-GFP mRNA. Scale bars, 5 μm.
Pcdna3 Ha Sam68 Wt Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sam68+wt+lentivirus+expression+vector/bio_rxiv__2022__10__24__513449-197-13-15?v=Addgene+inc
Average 93 stars, based on 1 article reviews
pcdna3 ha sam68 wt vector - by Bioz Stars, 2026-08
93/100 stars
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lbcas12a e795l expression vector pypq230 e795l  (Addgene inc)
93
Addgene inc lbcas12a e795l expression vector pypq230 e795l
a) Schematic illustration of the construct used for expressing RhoBAST-tagged G FP mRNA. b) Confocal images of HEK293T cells expressing GFP-RhoBAST 16 or GFP (control) mRNA incubated with SpyRho (100 nM) for 1 h before imaging. c) Normalized fluorescence profile along the dashed line in panel b (lower le). d) Schematic illustration of the constructs used for expressing CGG-containing FMR1-GFP mRNAs fused to 16 repeats of RhoBAST and <t>HaloTag7-Sam68.</t> e) Confocal images of live Cos7 cells expressing CGG99-FMR1-GFP-RhoBAST 16, CGG99-FMR1-GFP or GFP (control) mRNA and HaloTag7-Sam68 fusion protein (plasmid ratio 10:1) incubated with SpyRho (100 nM) and MaP700-Halo (200 nM, SiR channel) for 1 h before imaging. f) Normalized fluorescence profiles along the dashed lines shown in e). The fluorescence in the TMR channel was normalized to the highest value detected for CGG99-FMR1-GFP mRNA. Scale bars, 5 μm.
Lbcas12a E795l Expression Vector Pypq230 E795l, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sam68+wt+lentivirus+expression+vector/pmc08842731-62-15-19?v=Addgene+inc
Average 93 stars, based on 1 article reviews
lbcas12a e795l expression vector pypq230 e795l - by Bioz Stars, 2026-08
93/100 stars
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his-tag sam68 n-terminal region (amino acids 1–96  (GenScript corporation)
90
GenScript corporation his-tag sam68 n-terminal region (amino acids 1–96
a) Schematic illustration of the construct used for expressing RhoBAST-tagged G FP mRNA. b) Confocal images of HEK293T cells expressing GFP-RhoBAST 16 or GFP (control) mRNA incubated with SpyRho (100 nM) for 1 h before imaging. c) Normalized fluorescence profile along the dashed line in panel b (lower le). d) Schematic illustration of the constructs used for expressing CGG-containing FMR1-GFP mRNAs fused to 16 repeats of RhoBAST and <t>HaloTag7-Sam68.</t> e) Confocal images of live Cos7 cells expressing CGG99-FMR1-GFP-RhoBAST 16, CGG99-FMR1-GFP or GFP (control) mRNA and HaloTag7-Sam68 fusion protein (plasmid ratio 10:1) incubated with SpyRho (100 nM) and MaP700-Halo (200 nM, SiR channel) for 1 h before imaging. f) Normalized fluorescence profiles along the dashed lines shown in e). The fluorescence in the TMR channel was normalized to the highest value detected for CGG99-FMR1-GFP mRNA. Scale bars, 5 μm.
His Tag Sam68 N Terminal Region (Amino Acids 1–96, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sam68+wt+lentivirus+expression+vector/pm36537190-45-16-39?v=GenScript+corporation
Average 90 stars, based on 1 article reviews
his-tag sam68 n-terminal region (amino acids 1–96 - by Bioz Stars, 2026-08
90/100 stars
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pcdna3-ha-sam68 vector  (GenScript corporation)
90
GenScript corporation pcdna3-ha-sam68 vector
a) Schematic illustration of the construct used for expressing RhoBAST-tagged G FP mRNA. b) Confocal images of HEK293T cells expressing GFP-RhoBAST 16 or GFP (control) mRNA incubated with SpyRho (100 nM) for 1 h before imaging. c) Normalized fluorescence profile along the dashed line in panel b (lower le). d) Schematic illustration of the constructs used for expressing CGG-containing FMR1-GFP mRNAs fused to 16 repeats of RhoBAST and <t>HaloTag7-Sam68.</t> e) Confocal images of live Cos7 cells expressing CGG99-FMR1-GFP-RhoBAST 16, CGG99-FMR1-GFP or GFP (control) mRNA and HaloTag7-Sam68 fusion protein (plasmid ratio 10:1) incubated with SpyRho (100 nM) and MaP700-Halo (200 nM, SiR channel) for 1 h before imaging. f) Normalized fluorescence profiles along the dashed lines shown in e). The fluorescence in the TMR channel was normalized to the highest value detected for CGG99-FMR1-GFP mRNA. Scale bars, 5 μm.
Pcdna3 Ha Sam68 Vector, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sam68+wt+lentivirus+expression+vector/pm34099657-202-1-16?v=GenScript+corporation
Average 90 stars, based on 1 article reviews
pcdna3-ha-sam68 vector - by Bioz Stars, 2026-08
90/100 stars
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plasmid expression vector  (OriGene)
92
OriGene plasmid expression vector
a) Schematic illustration of the construct used for expressing RhoBAST-tagged G FP mRNA. b) Confocal images of HEK293T cells expressing GFP-RhoBAST 16 or GFP (control) mRNA incubated with SpyRho (100 nM) for 1 h before imaging. c) Normalized fluorescence profile along the dashed line in panel b (lower le). d) Schematic illustration of the constructs used for expressing CGG-containing FMR1-GFP mRNAs fused to 16 repeats of RhoBAST and <t>HaloTag7-Sam68.</t> e) Confocal images of live Cos7 cells expressing CGG99-FMR1-GFP-RhoBAST 16, CGG99-FMR1-GFP or GFP (control) mRNA and HaloTag7-Sam68 fusion protein (plasmid ratio 10:1) incubated with SpyRho (100 nM) and MaP700-Halo (200 nM, SiR channel) for 1 h before imaging. f) Normalized fluorescence profiles along the dashed lines shown in e). The fluorescence in the TMR channel was normalized to the highest value detected for CGG99-FMR1-GFP mRNA. Scale bars, 5 μm.
Plasmid Expression Vector, supplied by OriGene, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sam68+wt+lentivirus+expression+vector/pmc10920828__42003_2024_5969_MOESM1_ESM-83-4-8?v=OriGene
Average 92 stars, based on 1 article reviews
plasmid expression vector - by Bioz Stars, 2026-08
92/100 stars
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ascas12a ultra expression vector pypq220 ultra  (Addgene inc)
93
Addgene inc ascas12a ultra expression vector pypq220 ultra
a) Schematic illustration of the construct used for expressing RhoBAST-tagged G FP mRNA. b) Confocal images of HEK293T cells expressing GFP-RhoBAST 16 or GFP (control) mRNA incubated with SpyRho (100 nM) for 1 h before imaging. c) Normalized fluorescence profile along the dashed line in panel b (lower le). d) Schematic illustration of the constructs used for expressing CGG-containing FMR1-GFP mRNAs fused to 16 repeats of RhoBAST and <t>HaloTag7-Sam68.</t> e) Confocal images of live Cos7 cells expressing CGG99-FMR1-GFP-RhoBAST 16, CGG99-FMR1-GFP or GFP (control) mRNA and HaloTag7-Sam68 fusion protein (plasmid ratio 10:1) incubated with SpyRho (100 nM) and MaP700-Halo (200 nM, SiR channel) for 1 h before imaging. f) Normalized fluorescence profiles along the dashed lines shown in e). The fluorescence in the TMR channel was normalized to the highest value detected for CGG99-FMR1-GFP mRNA. Scale bars, 5 μm.
Ascas12a Ultra Expression Vector Pypq220 Ultra, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sam68+wt+lentivirus+expression+vector/pmc08842731-62-22-27?v=Addgene+inc
Average 93 stars, based on 1 article reviews
ascas12a ultra expression vector pypq220 ultra - by Bioz Stars, 2026-08
93/100 stars
  Buy from Supplier

Image Search Results


a) Schematic illustration of the construct used for expressing RhoBAST-tagged G FP mRNA. b) Confocal images of HEK293T cells expressing GFP-RhoBAST 16 or GFP (control) mRNA incubated with SpyRho (100 nM) for 1 h before imaging. c) Normalized fluorescence profile along the dashed line in panel b (lower le). d) Schematic illustration of the constructs used for expressing CGG-containing FMR1-GFP mRNAs fused to 16 repeats of RhoBAST and HaloTag7-Sam68. e) Confocal images of live Cos7 cells expressing CGG99-FMR1-GFP-RhoBAST 16, CGG99-FMR1-GFP or GFP (control) mRNA and HaloTag7-Sam68 fusion protein (plasmid ratio 10:1) incubated with SpyRho (100 nM) and MaP700-Halo (200 nM, SiR channel) for 1 h before imaging. f) Normalized fluorescence profiles along the dashed lines shown in e). The fluorescence in the TMR channel was normalized to the highest value detected for CGG99-FMR1-GFP mRNA. Scale bars, 5 μm.

Journal: bioRxiv

Article Title: Fast-exchanging spirocyclic rhodamine probes for aptamer-based super-resolution RNA imaging

doi: 10.1101/2022.10.24.513449

Figure Lengend Snippet: a) Schematic illustration of the construct used for expressing RhoBAST-tagged G FP mRNA. b) Confocal images of HEK293T cells expressing GFP-RhoBAST 16 or GFP (control) mRNA incubated with SpyRho (100 nM) for 1 h before imaging. c) Normalized fluorescence profile along the dashed line in panel b (lower le). d) Schematic illustration of the constructs used for expressing CGG-containing FMR1-GFP mRNAs fused to 16 repeats of RhoBAST and HaloTag7-Sam68. e) Confocal images of live Cos7 cells expressing CGG99-FMR1-GFP-RhoBAST 16, CGG99-FMR1-GFP or GFP (control) mRNA and HaloTag7-Sam68 fusion protein (plasmid ratio 10:1) incubated with SpyRho (100 nM) and MaP700-Halo (200 nM, SiR channel) for 1 h before imaging. f) Normalized fluorescence profiles along the dashed lines shown in e). The fluorescence in the TMR channel was normalized to the highest value detected for CGG99-FMR1-GFP mRNA. Scale bars, 5 μm.

Article Snippet: The vector backbone and the HaloTag7 insert were amplified by PCR using the pcDNA3-HA-Sam68-WT vector (Addgene, plasmid #17690) and pcDNA5-FRT-Halo-SNAP-NLS plasmid (kindly provided by the Johnsson group, Heidelberg) as templates.

Techniques: Construct, Expressing, Incubation, Imaging, Fluorescence, Plasmid Preparation